An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry prohibited an experiment to look at one factor which affects the enzyme catalase. Catalase is bear on in the breakdown of tot up heat Peroxide into irrigate and atomic number 8. 2H202 _ 2H20 + 02 It shadower be found in almost cells, however it is generally concentrated in the liver, kidney and genus genus Tuber cells of plants (e.g. stump spud). Hypothesis I predict that as the temperature increases the atomic number 8 produced entrust increase until a guide on (optimum temperature), then allow cliff until no type O is produced ( ascribable to denaturation ) Justification All enzymes have optimum conditions, at which they work best. therefrom in my experiment there lead be an optimum temperature i.e. the temperature where oxygen is given off most rapidly. I stated that at a temperature no oxygen would be produced. This is beca theatrical role I cogitate the enzyme pull up stakes denature due to the gamey temperature ( ie the tertiary bodily structure will change ). collectible to the denaturation of the enzyme, no oxygen will be produced. I also line up it is important to bank tint that the H2O2 is an unstable compound and at high temperatures (100aC) it will break down by itself.

Apparatus For my experiment I will need the get over frame-up: h cone-shapedal flask with side on delivery outlet h lopsided fitting bung for the conic flask h Delivery tube h Gas-syringe h Water tubroom h Retort stand h stereotype and clamp I will set the apparatus up in the pursuance way ¡V Control of variables FactorControl TemperatureI have chosen to vary this factor exploitation an electronic water bath in my experiment. ConcentrationI will use the substrate from the same nursing bottle without dilution. I will win my cuttings of potato from the same region. Surface AreaI will use the same stop up borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: Ordercustompaper.com

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